What is 20x Tris-Acetate-SDS Running Buffer used for?

20x Tris-Acetate-SDS Running Buffer is primarily used for protein electrophoresis in molecular biology labs. It facilitates the separation of proteins based on their size during gel electrophoresis. The buffer's high ionic strength and unique composition help stabilize pH levels during the run, ensuring more precise and reproducible results. Researchers often utilize this buffer when analyzing protein samples, confirming protein purity, or assessing molecular weight for better understanding of protein behavior in processes like Western blotting.

How do you prepare the 1x working solution from 20x Tris-Acetate-SDS Running Buffer?

To prepare a 1x working solution from a 20x Tris-Acetate-SDS Running Buffer, simply dilute 1 part of the 20x buffer with 19 parts of distilled water. For example, if you need 500 mL of 1x solution, mix 25 mL of the 20x buffer with 475 mL of distilled water. This simple dilution effectively adjusts the buffer concentration for optimal performance in gel electrophoresis, helping to ensure precise protein separation and enhanced analysis.

What types of samples can be run using this buffer?

This buffer is ideal for a variety of protein samples such as purified proteins, cell lysates, or recombinant proteins. It is commonly used for analyzing proteins extracted from biological tissues, microorganisms, or cultured cells. By ensuring consistent pH stability and optimal ionic strength, it enables accurate size-based separation of various protein types during SDS-PAGE. This versatility makes it a staple in laboratories focusing on protein research, diagnostics, or biotechnology applications.

Is 20x Tris-Acetate-SDS Running Buffer suitable for Western blotting?

Yes, 20x Tris-Acetate-SDS Running Buffer is suitable for Western blotting applications. After proteins are separated by SDS-PAGE using this buffer, they can be transferred to a membrane for further analysis. The buffer's composition promotes effective protein migration and maintaining structural integrity, which is crucial for accurate detection later in the Western blotting process. Many researchers depend on it for reliable results in protein identification and quantification.

What storage conditions are recommended for this buffer?

20x Tris-Acetate-SDS Running Buffer should be stored at room temperature, away from direct sunlight and extreme temperature fluctuations. Proper storage helps maintain its chemical stability and effectiveness for electrophoresis. Additionally, always ensure that the bottle is tightly sealed to prevent contamination or evaporation of essential components. Adequate storage practices extend the buffer’s usability, making it ready for your experiments whenever needed.

What is the pH range of the 1x working solution?

The pH of the 1x working solution of 20x Tris-Acetate-SDS Running Buffer typically ranges between 7.2 and 8.4, depending on the specific formulation. This pH range is critical for maintaining the integrity of proteins during electrophoresis while ensuring optimal separation conditions. Many users find that this pH range supports a wide array of protein types, enhancing the reproducibility of their experimental outcomes.

Can this buffer be used for DNA electrophoresis as well?

No, 20x Tris-Acetate-SDS Running Buffer is specifically designed for protein electrophoresis. The presence of SDS in the buffer denatures protein structures, allowing them to be separated based on size. For DNA electrophoresis, a different buffer, such as TAE or TBE, is typically used as they do not denature nucleic acids and provide appropriate charge and stability for DNA fragments. It’s essential to choose the right buffer depending on your analysis needs.

What equipment do I need to use this running buffer?

To utilize 20x Tris-Acetate-SDS Running Buffer, you'll need basic equipment such as an electrophoresis unit, power supply, and gel casting trays. Additionally, gel electrophoresis apparatus like combs and measuring devices are advisable for precise setup. This equipment works in tandem to ensure an effective separation process. Proper preparation and setup using the right tools enhance the efficiency and accuracy of your protein electrophoresis experiments.

What are the safety precautions when using this buffer?

When using 20x Tris-Acetate-SDS Running Buffer, it's important to wear appropriate personal protective equipment (PPE) such as gloves, lab coats, and safety goggles. The buffer contains SDS, which can be irritating to the skin and eyes. Therefore, avoid direct contact, and always work in a well-ventilated area. Additionally, ensure proper lab protocols are followed for disposal of any waste or spillage to maintain a safe working environment.

Where can I buy 20x Tris-Acetate-SDS Running Buffer 500 ML in Bangladesh?

You can buy 20x Tris-Acetate-SDS Running Buffer 500 ML on Ubuy. Ubuy offers a wide selection of laboratory supplies, including running buffers, enabling you to find the right products for your scientific needs. Their platform also provides important details about the product specifications and usage to assist you in making an informed purchase.

How to Shop 20x Tris-Acetate-SDS Running Buffer (500 ML) Online From Ubuy?

It’s easy to shop 20x Tris-Acetate-SDS Running Buffer (500 ML) online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is 20x Tris-Acetate-SDS Running Buffer (500 ML) Available to Shop Online in Bangladesh?

Yes, at Ubuy Bangladesh this product is available for you to shop at a reasonable price. The 20x Tris-Acetate-SDS Running Buffer (500 ML) is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

20x Tris-Acetate-SDS Running Buffer (500 ML)

Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer.

Item #: 125660636

20x Tris-Acetate-SDS Running Buffer (500 ML)

Item #: 125660636

BDT 9424

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from US}

Availability : In stock

Imported from USA store

QTY:

Order now and get it around Wednesday, October 07

Secured transaction

Our Top Logistics Partners

Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer.

U-Care Warranty:

None

Select a Plan

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of US store (110-120). Recommended power converters Buy Now.

What Stands Out

High Purity

This running buffer is formulated with high-purity reagents, ensuring reliable and reproducible results in electrophoresis applications, making it ideal for both research laboratories and educational institutions.

Convenience Pack

The convenient 500 ML packaging allows for easy handling and storage, providing sufficient volume for multiple experiments while maintaining product stability and prolonging shelf life.

Versatile Application

Designed for Tris-Acetate-SDS systems, this running buffer supports a range of protein separation techniques, catering to diverse scientific needs and enhancing productivity in both small-scale and large-scale experiments.

Product Details

Brand Name	Generic
Model Number	TAS01-500ML
Number of Items	1
Manufacturer	Bioland Scientific LLC
Manufacturer Part Number	TAS01-500ML
Brand	Generic
Item Weight	1.1 lbs (500 grams)

Who Should Buy?

Molecular Biologists
Ideal for molecular biologists conducting SDS-PAGE experiments to analyze protein samples effectively.
Educational Institutions
Well-suited for labs and classrooms focusing on protein separation techniques in educational settings.
Protein Research
Beneficial for researchers studying protein interactions, structures, and molecular weight using electrophoresis.

Casual Hobbyists
Not suitable for casual users without proper lab equipment and knowledge of protein analysis.

Product Description

20x Tris-Acetate-SDS Running Buffer (500 ML)

Have any Query? Chat with us

Customer Questions & Answers

Question: How to Shop 20x Tris-Acetate-SDS Running Buffer (500 ML) Online From Ubuy?

Answer: It’s easy to shop 20x Tris-Acetate-SDS Running Buffer (500 ML) online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 20x Tris-Acetate-SDS Running Buffer (500 ML) Available to Shop Online in Bangladesh?

Answer: Yes, at Ubuy Bangladesh this product is available for you to shop at a reasonable price. The 20x Tris-Acetate-SDS Running Buffer (500 ML) is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

Buffers TAS01-500ML Editorial Review

No editorial reviews found

Customer Reviews & Ratings

5.0

1 customers ratings

5 Star

100%
4 Star

0%
3 Star

0%
2 Star

0%
1 Star

0%

Review this product

Share your thoughts with other customers

Product Price History

Important information

Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
Not all the products listed on Ubuy are for sale, as Ubuy is a global search engine. Products are subject to export/trade regulations.

BDT 9424

Order now and get it around Wednesday, October 07

QTY:

Secured transaction

PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.

Our Top Logistics Partners

Fastest cross-border delivery

Features & Benefits

Highly effective for protein separation in denatured states.
Optimized for large molecular weight proteins.
Can be used with both Tris-Acetate SDS and LDS Sample Buffers.
Compatible with Tris-Glycine Native Running Buffer for more effective native protein resolution.
Stable for one year when stored at 4°C.
Enhances the performance of Tris-Acetate gels significantly.

Ubuy Assurance

Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

20x Tris-Acetate-SDS Running Buffer (500 ML)

BDT 9424

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Product Details

Who Should Buy?

Product Description

Customer Questions & Answers

Buffers TAS01-500ML Editorial Review

Customer Reviews & Ratings

Review this product

Product Price History

Important information

BDT 9424

Features & Benefits

Ubuy Assurance

Fast
Shipping

Free
Return^*