What is HEPES, and what are its common applications?

HEPES, or N-2-Hydroxyethyl piperazine N'-2-ethanesulfonic Acid, is a buffering agent commonly used in biological and biochemical research. It maintains pH levels in cell culture and various biochemical experiments, making it essential for maintaining cell viability. HEPES is particularly beneficial in studies involving sensitive enzymes and molecular biology applications, such as protein purification and enzyme assays.

Is HEPES suitable for use in cell culture?

Yes, HEPES is highly suitable for cell culture applications. Its buffering capacity helps maintain stable pH conditions, which is critical for cell growth and function. Using HEPES in cell culture media can improve the performance of cells under various conditions, especially when pH fluctuations might occur, such as in high carbon dioxide environments.

How should HEPES be stored for optimal stability?

HEPES should be stored in a cool, dry place, away from light and moisture to ensure its stability. Typically, it is best kept in tightly sealed containers. Proper storage conditions help maintain the integrity of the compound, allowing researchers to achieve accurate and reliable results in experiments involving this important buffer.

Can HEPES be used in pharmaceutical formulations?

Yes, HEPES can be utilized in various pharmaceutical formulations as a buffering agent. Its ability to maintain a stable pH is beneficial in injectable solutions, helping to ensure drug efficacy. Additionally, HEPES may be used in the production of certain vaccines, enhancing the stability of active ingredients during formulation.

What concentration of HEPES should be used for effective buffering?

The effective concentration of HEPES for buffering typically ranges from 10 mM to 100 mM, depending on the specific requirements of the experiment. Researchers should select a concentration based on the pH stability needed and the nature of their biological system, tailoring it to suit their unique application.

Are there any safety precautions for handling HEPES?

When handling HEPES, standard laboratory safety practices should be followed. Wearing gloves, goggles, and a lab coat is advisable to prevent skin and eye contact. Additionally, ensuring proper ventilation while using HEPES in powder form can minimize inhalation exposure, thereby promoting a safe working environment.

What are alternative buffers to HEPES?

Although HEPES is widely used, alternatives include Tris (tris(hydroxymethyl)aminomethane), PBS (phosphate-buffered saline), and MES (2-(N-morpholino)ethanesulfonic acid). Each alternative may be preferable in different experimental conditions, depending on factors such as pH range and ionic strength requirements.

Can HEPES interfere with my experiments?

In general, HEPES is a stable buffer and is unlikely to interfere with most biochemical assays. However, it’s important to account for possible interactions with specific reagents or biological molecules. Conducting preliminary tests can help gauge its impact and ensure optimal results in your experimental setups.

What is the difference between HEPES and other common buffers?

HEPES differs from buffers like Tris and bicarbonate by having a broader effective pH range and greater buffering capacity at physiological pH. This makes it ideal for maintaining the pH in biological experiments. Additionally, HEPES remains effective at higher temperatures, which is beneficial in various laboratory conditions.

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HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic Acid)], 50 Grams

Product is sold for research or further manufacturing use only, not for food or drug use.

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Item #: 30780181

HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic Acid)], 50 Grams

Q: Where can I buy HEPES, Free Acid [N-2-Hydroxyethyl piperazine N'-2-ethanesulfonic Acid], 50 Grams?

You can buy HEPES, Free Acid [N-2-Hydroxyethyl piperazine N'-2-ethanesulfonic Acid], 50 Grams from Ubuy. They offer a diverse range of laboratory chemicals and equipment, providing a convenient option for researchers and laboratories looking to acquire quality reagents easily.

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What Stands Out

High Purity

This HEPES free acid offers exceptional purity, ensuring reliable performance in biochemical applications. It minimizes the risk of contamination and enhances experimental accuracy.

Versatile Buffer

Ideal for various biological experiments, this buffer works effectively across a wide pH range, making it a perfect choice for cell culture and enzymatic assays.

Convenient Packaging

Packaged in 50 grams, this product provides ample quantity for extensive research use while ensuring easy storage and handling, catering to both small-scale and large-scale laboratory needs.

Product Details

Country of Origin	This item will be imported from US
Item Weight	0.11 lbs (50 grams)
What is in the box	HEPES, Free Acid [N-(2-Hydroxyethyl)... For more details, please check description/product details

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Who Should Buy?

Cell Culture Experts
Ideal for biologists performing cell culture, providing stable pH conditions essential for accurate experimental outcomes.
Biochemical Research
Beneficial for researchers requiring reliable buffering agents in biochemical assays and experiments dealing with proteins.
Laboratory Settings
Perfect for laboratories needing quality buffer solutions for various scientific applications including microbiology and molecular biology.

Casual Users
Not suitable for individuals without a background in chemistry or biology who may mismanage the product.

Product Description

HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic Acid)], 50 Grams

About This Item

Introducing HEPES, Free Acid [N-2-Hydroxyethyl piperazine N-2-ethanesulfonic Acid], a versatile and essential laboratory buffer. Whether you're a researcher, scientist, or chemist, this product is designed to meet your needs. HEPES is a zwitterionic N-substituted aminosulfonic acid buffer, providing exceptional pH stability for a wide range of applications.

With a pKa of 7.5 (at 25°C) and a pH range of 6.8 to 8.2 for working solutions, HEPES ensures accurate and consistent results every time. This 50-gram container of HEPES Free Acid is perfect for those looking to stock up on this essential lab reagent. As a trusted supplier on Ubuy, we understand the importance of reliable products and guarantee the quality and purity of our HEPES Free Acid. Our easy-to-use e-commerce platform allows you to conveniently place your order from the comfort of your home or office.

You can trust our retail website to provide a seamless online shopping experience, offering a wide variety of laboratory reagents and supplies. When you choose Ubuy as your preferred online marketplace, you gain access to a user-friendly interface, secure payment options, and prompt delivery right to your doorstep. We strive to make the process of purchasing online simple and hassle-free. Don't waste any more time searching for the right lab buffer. Ubuy is your go-to online store for all your scientific needs.

Shop with confidence on our e-commerce site, where quality, reliability, and customer satisfaction are our top priorities. Experience the convenience of online shopping with Ubuy. Order your HEPES, Free Acid [N-2-Hydroxyethyl piperazine N-2-ethanesulfonic Acid] today and streamline your laboratory processes like never before.

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Question: How to Shop HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine Online From Ubuy?

Answer: It’s easy to shop HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine Available to Shop Online in Bangladesh?

Answer: Yes, at Ubuy Bangladesh this product is available for you to shop at a reasonable price. The HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

RPI Buffers Editorial Review

HEPES, Free Acid is a highly recommended product in the scientific community. It is often used as a buffering agent in biological research. The product comes in a 50 gram quantity which is sufficient for many experiments. It is a high-quality HEPES powder that dissolves easily in water, providing accurate and reliable results. The purity of the product is excellent and it is packed securely to ensure its integrity during shipping. Overall, HEPES, Free Acid is a reliable and effective product for any laboratory.

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Highly recommended in the scientific community
Sufficient quantity for many experiments
High-quality powder that dissolves easily
Excellent purity
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Features & Benefits

Zwitterionic N-substituted aminosulfonic acid buffer
pKa (25°C): 7.5
pH Range (Working): 6.8 - 8.2
Heavy Metals free
Chemical Formula: C8H18N2O4S
CAS Number: 7365-45-9

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HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic Acid)], 50 Grams

BDT 20846

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Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

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What Stands Out

Product Details

Dangerous Goods

Who Should Buy?

Product Description

About This Item

Customer Questions & Answers

RPI Buffers Editorial Review

Customer Reviews & Ratings

Review this product

Pros

Product Price History

Important information

BDT 20846

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