HiMedia PT022-5L Murashige and Skoog Shoot Multiplication Medium A with CaCl2, Vitamins, Sucrose, IAA, Adenine Sulphate and 2iP, 5 L
BDT 9690
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Ideal for plant tissue culture with essential nutrients for shoot multiplication.
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What Stands Out
Product Details
- Plant Tissue Culture
- Package Weight : 0.068 pounds
- Made in India
- Package Dimensions : 0.15 L x 0.35 H x 0.15 W (inches)
Product Technical Details
| Package Dimensions | 5.79 x 3.58 x 3.5 inches |
| Item Weight | 14 ounces |
| Manufacturer | HiMedia Laboratories |
| Item model number | PT022-5L |
Who Should Buy?
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Plant Researchers
Ideal for scientists conducting plant tissue culture experiments and needing a reliable growth medium for shoot multiplication.
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Agricultural Technologists
Beneficial for agricultural professionals focusing on crop improvement and propagation strategies using tissue culture techniques.
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Educational Institutions
Perfect for universities and colleges offering biotechnology courses, enabling hands-on training in plant tissue culture.
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Home Gardeners
Not suitable for casual gardeners who may find corporate-grade mediums unnecessary for standard gardening practices.
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Hobbyist Growers
Less appropriate for amateur enthusiasts who prefer simpler, ready-to-use solutions without complex formulations.
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Non-Plant Studies
Not ideal for individuals or projects that do not involve plant tissue culture or agricultural research.
Product Description
Customer Questions & Answers
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Question:
What is HiMedia PT022-5L Murashige and Skoog Shoot Multiplication Medium A used for?
Answer: HiMedia PT022-5L Murashige and Skoog Shoot Multiplication Medium A is primarily used for plant tissue culture, particularly for the shoot multiplication stage. This medium is rich in nutrients, vitamins, and hormones, which support the growth and development of plant tissues in sterile conditions. It is suitable for various plant species, making it suitable for research and commercial propagation. Laboratories and horticulturists utilize this medium to enhance the yield and health of plant clones. -
Question:
What are the key components of the Murashige and Skoog medium?
Answer: The key components of the HiMedia PT022-5L Murashige and Skoog medium include essential nutrients, CaCl2 (calcium chloride), vitamins, sucrose, IAA (Indole-3-acetic acid), adenine sulphate, and 2iP (a cytokinin). These ingredients play a crucial role in supporting plant cell division and differentiation, essential for shoot multiplication. The balanced combination of these components creates an optimal environment for plant growth and regeneration, making it essential for tissue culture practices. -
Question:
Is the HiMedia Murashige and Skoog medium suitable for all plant species?
Answer: While the HiMedia Murashige and Skoog medium is designed to support a wide range of plant species, its effectiveness can vary based on the specific requirements of each plant. For many common species, this medium works exceptionally well; however, some species may require specific modifications or additional supplements. Researchers often experiment with this medium for optimal results, making it highly adaptable for numerous plant tissue culture applications. -
Question:
How should I prepare the HiMedia PT022-5L medium for use?
Answer: To prepare the HiMedia PT022-5L Murashige and Skoog medium, first, dissolve the concentrated medium in distilled or deionized water according to the recommended protocol. Adjust the pH to the desired level, typically around 5.7 to 5.8, using either NaOH or HCl. Finally, autoclave the medium for sterilization before use in tissue culture experiments. Proper preparation ensures that the medium provides a nutrient-rich environment conducive to plant growth. -
Question:
What benefits does the addition of CaCl2 provide in the medium?
Answer: The inclusion of CaCl2 (calcium chloride) in the HiMedia Murashige and Skoog medium helps in maintaining cellular integrity and encourages cell division. Calcium ions play a critical role in cell wall stabilization and the regulation of various physiological processes within the plant cells. By providing these essential calcium ions, the medium fosters healthier shoot multiplication and improves overall plant development, contributing to successful tissue culture outcomes. -
Question:
Can I store the prepared HiMedia Murashige and Skoog medium?
Answer: Yes, the prepared HiMedia Murashige and Skoog medium can be stored under proper conditions to prolong its usability. It is best to keep the prepared medium in a sterile container and under refrigerated conditions if it is not used immediately. However, for optimal results, it is advisable to use the medium within a few weeks. Proper storage helps in maintaining the effectiveness of the nutrients and hormones essential for plant growth. -
Question:
What is the role of sucrose in the HiMedia medium?
Answer: Sucrose in the HiMedia PT022-5L Murashige and Skoog medium serves as a primary carbon source for plant tissues, providing essential energy for growth. Its presence is vital, as plant cells utilize sucrose for respiration, facilitating metabolic activities and promoting shoot multiplication. The concentration of sucrose can be adjusted based on the specific needs of the plants being cultured, enhancing their growth performance and vigor. -
Question:
How does the inclusion of IAA benefit plant tissue cultures?
Answer: IAA (Indole-3-acetic acid) is a naturally occurring plant hormone known for its role in supporting cell elongation and division. In the HiMedia Murashige and Skoog medium, the presence of IAA encourages the development of roots and shoots in tissue cultures. It plays a critical role in promoting overall plant growth, making it an essential component for successful plant propagation and regeneration in laboratory settings. -
Question:
What are practical applications of using the HiMedia PT022-5L medium?
Answer: The HiMedia PT022-5L Murashige and Skoog medium is utilized extensively in laboratories for plant tissue culture projects, including research, plant breeding, and conservation efforts. Horticulturists use it for mass propagation of high-value crops, ornamental plants, and rare species, ensuring higher yields and better plant health. This medium's versatile applications make it an essential tool in modern plant biology and agriculture. -
Question:
Where can I buy HiMedia PT022-5L Murashige and Skoog Shoot Multiplication Medium A with CaCl2, Vitamins, Sucrose, IAA, Adenine Sulphate and 2iP in Bangladesh?
Answer: You can buy the HiMedia PT022-5L Murashige and Skoog Shoot Multiplication Medium A from Ubuy in Bangladesh. Ubuy offers a wide selection of laboratory supplies, including this specific medium, making it convenient for researchers and horticulturists to find quality products. Shopping on Ubuy ensures you can access this essential medium with ease.
HiMedia Analytical Reagents PT022-5L Editorial Review
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BDT 9690
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Features & Benefits
- Formulated specifically for shoot multiplication in plant tissue culture.
- Contains vital nutrients including CaCl2, vitamins, and sucrose.
- Enhanced with IAA, adenine sulphate, and 2iP for effective growth.
- Packaged in a convenient 5L size for extensive use.
- Lightweight at only 0.068 pounds for easy handling.
- Manufactured in India ensuring quality and reliability.
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