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Item #:
27675834
Phosphate Buffered Saline, PBS (10X Concentrated), Sterile, pH 7.4, 500 mL
Item #:
27675834
BDT 10428
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Our PBS formulation does not contain calcium and magnesium, divalent ions that stimulate cell aggregation.
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What Stands Out
Concentration
This 10X concentrated PBS requires dilution, ensuring cost-effectiveness while providing precise buffering capacity for various laboratory applications.
Sterile Packaging
Sterile formulation guarantees contamination-free experiments, making it ideal for sensitive biological research and maintaining accuracy in results.
pH Control
With a stable pH of 7.4, this solution offers optimal conditions for cellular activities, enhancing experiment reliability and reproducibility.
Product Details
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Laboratory Researchers
Essential for cellular and molecular biology experiments, providing a stable pH and osmotic environment.
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Cell Culture
Ideal for maintaining and transporting cells in vitro, ensuring optimal growth conditions.
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Biochemical Applications
Useful for diluting proteins and other reagents in various biochemical assays and experiments.
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Food Preparation
Not suitable for human consumption or any food-related applications due to its chemical composition.
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Non-sterile Settings
Not appropriate for environments where sterility cannot be maintained, risking contamination.
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Personal Care Products
Not designed for cosmetic or skincare formulations, as it may cause adverse effects on the skin.
Product Description
Phosphate Buffered Saline, PBS (10X Concentrated), Sterile, pH 7.4, 500 mL
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is Phosphate Buffered Saline (PBS) 10X Concentrated?
Answer: Phosphate Buffered Saline (PBS) 10X Concentrated is a buffer solution commonly used in biological research and cell culture. This sterile solution maintains a stable pH of 7.4, making it ideal for maintaining the physiological pH of cells. It is formulated with phosphates that help to regulate osmotic pressure, enabling optimal cell growth and preservation. For instance, it's often used when washing cells, diluting samples, or as a diluent for various laboratory reagents. -
Question:
What are the applications of PBS 10X in laboratories?
Answer: PBS 10X is widely used in laboratories for various applications, including cell culture, immunoassays, and molecular biology. It serves as a washing buffer in immunofluorescence and other assay procedures to remove unbound reagents while keeping cells viable. Additionally, it can be diluted to a working concentration of 1X for use in applications like sample preparation and diluting tissue or blood samples for analysis. Its versatility makes it an essential component for researchers across multiple fields. -
Question:
How do I prepare a 1X solution from PBS 10X Concentrated?
Answer: To prepare a 1X solution from PBS 10X Concentrated, you need to dilute the concentrated solution with distilled water. For every 1 part of PBS 10X, mix it with 9 parts of distilled water to achieve a final concentration of 1X. This method ensures that the buffer retains its pH and ionic strength, which is critical for experiments. For example, if you need 100 mL of 1X PBS, mix 10 mL of PBS 10X with 90 mL of distilled water. -
Question:
Is PBS 10X suitable for sensitive experiments?
Answer: Yes, PBS 10X is suitable for sensitive experiments, especially when maintaining a stable pH is essential. The formulation is designed to avoid interfering with biochemical assays and provides a physiologically relevant environment for cells or tissues. Researchers often use PBS in experiments involving sensitive reagents, such as antibodies or enzymes, to ensure minimal background noise and reliable results. Always ensure that the PBS is sterile, especially when working with primary cells or complex assays. -
Question:
How should I store Phosphate Buffered Saline?
Answer: Phosphate Buffered Saline should be stored at room temperature or in a refrigerator, depending on the manufacturer’s recommendations. Keep the bottle tightly closed and protected from light to prevent degradation. For long-term use, avoid repeated freeze-thaw cycles, as they can alter the buffer’s properties. In practical terms, this means you can store it for use over an extended period while ensuring the integrity of your samples during critical experiments. -
Question:
Can I use PBS 10X for cell culture?
Answer: Absolutely, PBS 10X is frequently used for cell culture practices. It acts as a rinse buffer during cell washing steps, helping to maintain cell viability while removing debris and excess media components. It is especially useful when preparing cells for assays or when resuspending cells after trypsinization. For best results, always dilute it to a 1X concentration before use in cell culture procedures to ensure optimal conditions for cell growth. -
Question:
What is the pH of PBS and why is it important?
Answer: PBS has a pH of 7.4, which is essential for mimicking the physiological conditions of human and animal cells. Maintaining a proper pH is crucial as it affects enzyme activity, protein stability, and overall cellular health. This characteristic makes PBS an optimal medium for experiments that require accurate biological fidelity. For example, pH stability is particularly important in enzymatic reactions or when working with cell lines sensitive to pH fluctuations. -
Question:
Is PBS 10X safe to use in biological applications?
Answer: Yes, PBS 10X is considered safe for use in biological applications as it is non-toxic and does not possess harmful ingredients that could affect biological samples. As a sterile solution, it minimizes the risk of contamination during experiments. It is widely utilized in various domains such as microbiology, immunology, and molecular biology, ensuring researchers can confidently conduct their experiments without adverse effects on cell cultures or biomolecules. -
Question:
Where can I buy Phosphate Buffered Saline, PBS 10X Concentrated, Sterile, pH 7.4, 500 mL?
Answer: You can buy Phosphate Buffered Saline, PBS 10X Concentrated, Sterile, pH 7.4, 500 mL from Ubuy in Bangladesh. Ubuy offers a reliable platform for purchasing quality laboratory supplies, making it a great option for researchers and lab professionals looking for essential reagents like PBS. -
Question:
Are there different formulations of PBS available?
Answer: Yes, PBS formulations can vary widely to meet specific laboratory needs. While the standard PBS contains phosphates for buffering and sodium chloride to maintain osmolarity, some formulations may include calcium or magnesium for use in cell culture applications. Additionally, there are variations pH-adjusted for specialized assays. Researchers often select the formulation based on their unique experimental requirements, ensuring compatibility with their specific biological systems.
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